454 sequencing of Barley cv. Morax fragment library - SRA

SRX004996: 454 sequencing of Barley cv. Morax fragment library
1 LS454 (454 GS 20) run: 13,422 spots, 1.5M bases, 3.6Mb downloads

Design: Preparation and sequencing of the 454 sequencing library was essentially performed according to manufactorer’s instructions (GS20 Shotgun DNA Library Preparation Kit / Sequencing Kit, Roche Diagnostics, March 2007). About 4 µg of barley cv. Morex whole genome DNA were fragmented for 1 min at 3 bar N2 by nebulizers to an average fragment length of 700 bp (determined on DNA-1000 LabChip, Agilent). The library was quantified by RNA-1000 LabChip (Agilent) and the Quant-IT RiboGreen RNA assay (Invitrogen). The reads analysed in this study were generated on a GS20 by two runs (two segments each) with 70x75 picotiterplates and 4 out of 8 segments ("titration") of a 40x75 picotiterplate.

Submitted by: Genome Analysis in the Biological System Plant (GABIPD)

Study: Low-pass shotgun sequencing of the barley genome facilitates rapid identification of genes, conserved non-coding sequences and novel repeats

• SRP000229 • All experiments • All runs

show Abstracthide Abstract

Barley has one of the largest and most complex genomes of all economically important food crops. The rise of new short read sequencing technologies such as Illumina/Solexa permits such large genomes to be effectively sampled at relatively low cost. Based on the corresponding sequence reads a Mathematically Defined Repeat (MDR) index can be generated to map repetitive regions in genomic sequences.

Sample: Generic sample from Hordeum vulgare

SAMN00011084 • SRS000573 • All experiments • All runs

Organism: Hordeum vulgare

Library:

Name: 2007_06_01_T1

Instrument: 454 GS 20

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 13,422 spots, 1.5M bases, 3.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR016817	13,422	1.5M	3.6Mb	2009-10-06

ID:: 5252

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